pEGFP-transfected cells

        Control               E+/B48                   E‾/B48

S51A-transfected cells

       Control                E+/B48              E‾/B48       

Total RNAa

LAL

0.15 ± 0.02

  0.21 ± 0.03*

0.18 ± 0.03

0.18 ± 0.02

0.20 ± 0.03

0.22 ± 0.03

Cath B

0.53 ± 0.04

  0.69 ± 0.09*

0.57 ± 0.07

0.66 ± 0.09

0.67 ±0.09

0.72 ± 0.08

ATF4

0.20 ± 0.02

  0.21 ± 0.03

0.23 ± 0.05

0.18 ± 0.03

0.17 ± 0.03

0.19 ± 0.03

Polysome-associated RNAb

LAL

0.29 ± 0.02

  0.26 ± 0.02

0.21 ± 0.03*

0.29 ± 0.06

0.31 ± 0.04

0.32 ± 0.04†

Cath B

0.24 ± 0.02

  0.25 ± 0.04

0.17 ± 0.02*

0.27 ± 0.02

0.26 ± 0.01

0.28 ± 0.01†

ATF4

0.63 ± 0.12

  0.64 ± 0.05

0.91 ± 0.11*

0.69 ± 0.11

0.61 ± 0.09

0.73 ± 0.10†


LAL; lysosomal acid lipase, Cath B; cathepsin B, ATF4; activating transcription factor 4
aSteady-state level of each mRNA was expressed relative to GAPDH mRNA levels.
bPolysome-associated mRNA levels were normalized with respect to its steady-state
level.
Values represent the mean ± SEM of five experiments.
* P<0.05 as compared with the control, and † P<0.05 as compared with the cells transfected with empty pEGFP vector and treated with EÎ/B48 lipoproteins.
Table 1: Differential regulation of steady-state levels and translational efficiencies of specific mRNAs in Raw 264.7 cells treated with E‾/B48 lipoproteins.
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