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Figure 1: (A) Western blot analysis of γ-tocotrienol and celecoxib treatment effects on PGDH in neoplastic +SA mammary epithelial cells. Cells were initially plated at a density of 1x106 cells/100 mm culture dishes, divided into different treatment groups, and then maintained on their respective control or treatment media for a 4-day treatment period. Afterwards, cells were isolated and prepared for Western blot analysis. Samples were analyzed for relative levels of the PGDH. Scanning densitometric analysis was performed for each blot to visualize the relative levels of proteins. Integrated optical density of each band was normalized with their corresponding β-actin and control treatment bands and then shown in bar graphs. Vertical bars indicate the fold-change in protein levels in various treatment groups as compared with their respective controls. (B) Effects of celecoxib and γ-tocotrienol treatment alone and in combination on PGE2 synthesis in neoplastic +SA mammary epithelial cells. Cells were initially plated at a density of 5x104 cells/well in 24-well culture plates, divided into the different treatment groups, and then maintained on their respective control or treatment media for a 72 hr treatment period. Afterward, media was collected from the different treatment groups and prepared for use in the EIA assay for PGE2. Vertical bars indicate the mean cell count + SEM in each treatment group. *P<0.05 as compared with the vehicle-treated control group.
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