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Figure 6: Scores Plots in 3D (3 PCs retained) or 2D (2 PCs retained) for the 4 normalization methods promoted to principal components analysis plus the raw data. The timepoints of the experiment were grouped into phases: timepoint 1 (baseline; black circles), timepoint 2 (Shock 45; red triangles), early resuscitation (timepoints 3-5; green crosses), late resuscitation (timepoints 6-9; blue x), and experimental endpoint (timepoints 10-11; cyan diamonds). (6A): Raw urine metabolomics data expressed in 3 PC variables, retaining 15 metabolites and 66% of the variation. (6B) Urine metabolomics data normalized to CS-LGU expressed in 3 PC variables. Twenty metabolites and 41% of the variation are retained. (6C) Urine metabolomics data normalized to PQ2 expressed in 2 PC variables. Thirteen metabolites and 53% of the variation are retained. (6D) Urine metabolomics data normalized to OSM expressed in 2 PC variables. Sixteen metabolites and 35% of the variation are retained. (6E) Urine metabolomics data normalized to UO expressed in 2 PC variables. Twelve metabolites and 54% of the variation are retained.
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