Calibration
Linearity range 0.05 - 5 ng/mL
Slope ± S.D. (n=5) 0.97633 ± 0.03495
C.V.% (slope) 3.6
r (mean ± S.D) 0.99984 ± 0.00016
Intercept (mean ± S.D) 0.01256 ± 0.00458
Limit of quantification (LOQ) 0.05 ng/mL
Limit of detection (LOD) 0.003 ng/mL
Intra-assay precision and accuracy (n=5)
Conc. (ng/mL) Measured mean (ng/mL) ± S.D. C.V.(%) Mean accuracy (%)
0.05 0.04902 ± 0.0024 4.9 98
0.10 0.09542 ± 0.0029 3.1 96
0.25 0.22957 ± 0.0052 2.2 92
1.0 0.88797 ± 0.0205 2.1 89
2.5 2.23212 ± 0.0379 1.5 89
Inter-assay precision and accuracy (n=5)
Conc. (ng/mL) Measured mean (ng/mL) ± S.D. C.V.(%) Mean accuracy (%)
0.10 0.10009 ± 0.00948 9.4 101
0.25 0.24932 ± 0.01437 5.8 100
1.0 0.93760 ± 0.04746 5.1 94
2.5 2.32050 ± 0.08518 3.7 93
Table 1: Method validation data.
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