Biological sample

 

Intraday

Interday

Nominal (µg/ml)

Obtained (µg/ml)

Accuracy (%)

% CV

Obtained (µg/ml)

Accuracy (%)

% CV

Plasma

LLOQ

0.25

0.259

103.71

1.86

0.256

102.51

3.33

LQC

0.30

0.318

105.89

2.97

0.312

104.07

3.61

MQC

0.90

0.967

107.40

1.95

0.920

102.23

3.80

HQC

5.00

4.792

95.84

1.48

4.987

100.00

3.05

Blood

LLOQ

0.25

0.248

99.15

0.90

0.251

100.21

1.22

LQC

0.30

0.296

98.77

1.50

0.299

99.53

2.57

MQC

0.70

0.698

99.69

0.91

0.700

100.07

0.94

HQC

3.00

3.045

101.50

1.58

3.023

100.92

1.38

Lung

LLOQ

0.40

0.407

101.70

1.60

0.400

100.12

2.21

LQC

0.50

0.500

100.06

1.25

0.502

100.36

1.15

MQC

0.90

0.901

100.09

1.83

0.902

100.20

1.00

HQC

3.00

3.022

100.73

1.68

3.044

101.40

1.39

Liver

LLOQ

0.60

0.618

103.08

1.23

0.604

100.59

1.92

LQC

0.70

0.691

98.74

1.39

0.699

99.83

1.14

MQC

1.50

1.502

100.11

1.42

1.501

100.03

0.71

HQC

5.00

4.976

99.53

1.21

5.012

100.43

1.15

Heart

LLOQ

0.40

0.399

99.84

1.26

0.402

100.42

1.62

LQC

0.50

0.492

98.46

1.44

0.496

99.23

2.14

MQC

1.50

1.499

99.94

1.14

1.500

100.00

0.90

HQC

5.00

4.922

98.44

2.04

4.949

99.21

1.57

Small intestine

LLOQ

0.60

0.608

101.39

1.25

0.592

98.67

2.95

LQC

0.70

0.681

97.31

1.41

0.688

98.34

1.83

MQC

1.50

1.493

99.55

1.43

1.493

99.51

1.16

HQC

5.00

4.975

99.50

1.21

5.029

100.69

1.13

Large intestine

LLOQ

0.25

0.261

103.71

0.86

0.251

100.05

2.78

LQC

0.30

0.309

103.01

1.94

0.301

100.26

2.98

MQC

0.70

0.752

107.48

0.90

0.717

102.40

4.46

HQC

3.00

3.071

103.66

1.25

3.038

101.61

1.78

Brain

LLOQ

0.25

0.238

95.29

1.30

0.246

98.23

2.88

LQC

0.30

0.303

101.05

6.22

0.298

99.36

4.83

MQC

0.70

0.697

99.60

1.75

0.699

99.87

2.16

HQC

1.50

1.497

99.81

3.69

1.498

99.99

2.09

Stomach

LLOQ

0.25

0.248

99.15

0.90

0.250

99.97

1.61

LQC

0.30

0.296

98.77

1.50

0.299

99.66

2.25

MQC

0.70

0.698

99.69

0.91

0.701

100.11

0.93

HQC

3.00

3.045

101.50

1.58

3.010

100.16

1.97

Accuracy (%), [(Obtained concentration/nominal concentration) x 100]; CV (%), coefficient of variation [(S.D./mean) x 100].
Table 2: Intraday and inter day precision and accuracy of BITS 17 from Biological samples.
Goto home»