Biological sample |
|
Intraday |
Interday |
Nominal
(µg/ml) |
Obtained
(µg/ml) |
Accuracy
(%) |
% CV |
Obtained
(µg/ml) |
Accuracy
(%) |
% CV |
Plasma |
LLOQ |
0.25 |
0.259 |
103.71 |
1.86 |
0.256 |
102.51 |
3.33 |
|
LQC |
0.30 |
0.318 |
105.89 |
2.97 |
0.312 |
104.07 |
3.61 |
|
MQC |
0.90 |
0.967 |
107.40 |
1.95 |
0.920 |
102.23 |
3.80 |
|
HQC |
5.00 |
4.792 |
95.84 |
1.48 |
4.987 |
100.00 |
3.05 |
Blood |
LLOQ |
0.25 |
0.248 |
99.15 |
0.90 |
0.251 |
100.21 |
1.22 |
|
LQC |
0.30 |
0.296 |
98.77 |
1.50 |
0.299 |
99.53 |
2.57 |
|
MQC |
0.70 |
0.698 |
99.69 |
0.91 |
0.700 |
100.07 |
0.94 |
|
HQC |
3.00 |
3.045 |
101.50 |
1.58 |
3.023 |
100.92 |
1.38 |
Lung |
LLOQ |
0.40 |
0.407 |
101.70 |
1.60 |
0.400 |
100.12 |
2.21 |
|
LQC |
0.50 |
0.500 |
100.06 |
1.25 |
0.502 |
100.36 |
1.15 |
|
MQC |
0.90 |
0.901 |
100.09 |
1.83 |
0.902 |
100.20 |
1.00 |
|
HQC |
3.00 |
3.022 |
100.73 |
1.68 |
3.044 |
101.40 |
1.39 |
Liver |
LLOQ |
0.60 |
0.618 |
103.08 |
1.23 |
0.604 |
100.59 |
1.92 |
|
LQC |
0.70 |
0.691 |
98.74 |
1.39 |
0.699 |
99.83 |
1.14 |
|
MQC |
1.50 |
1.502 |
100.11 |
1.42 |
1.501 |
100.03 |
0.71 |
|
HQC |
5.00 |
4.976 |
99.53 |
1.21 |
5.012 |
100.43 |
1.15 |
Heart |
LLOQ |
0.40 |
0.399 |
99.84 |
1.26 |
0.402 |
100.42 |
1.62 |
|
LQC |
0.50 |
0.492 |
98.46 |
1.44 |
0.496 |
99.23 |
2.14 |
|
MQC |
1.50 |
1.499 |
99.94 |
1.14 |
1.500 |
100.00 |
0.90 |
|
HQC |
5.00 |
4.922 |
98.44 |
2.04 |
4.949 |
99.21 |
1.57 |
Small intestine |
LLOQ |
0.60 |
0.608 |
101.39 |
1.25 |
0.592 |
98.67 |
2.95 |
|
LQC |
0.70 |
0.681 |
97.31 |
1.41 |
0.688 |
98.34 |
1.83 |
|
MQC |
1.50 |
1.493 |
99.55 |
1.43 |
1.493 |
99.51 |
1.16 |
|
HQC |
5.00 |
4.975 |
99.50 |
1.21 |
5.029 |
100.69 |
1.13 |
Large intestine |
LLOQ |
0.25 |
0.261 |
103.71 |
0.86 |
0.251 |
100.05 |
2.78 |
|
LQC |
0.30 |
0.309 |
103.01 |
1.94 |
0.301 |
100.26 |
2.98 |
|
MQC |
0.70 |
0.752 |
107.48 |
0.90 |
0.717 |
102.40 |
4.46 |
|
HQC |
3.00 |
3.071 |
103.66 |
1.25 |
3.038 |
101.61 |
1.78 |
Brain |
LLOQ |
0.25 |
0.238 |
95.29 |
1.30 |
0.246 |
98.23 |
2.88 |
|
LQC |
0.30 |
0.303 |
101.05 |
6.22 |
0.298 |
99.36 |
4.83 |
|
MQC |
0.70 |
0.697 |
99.60 |
1.75 |
0.699 |
99.87 |
2.16 |
|
HQC |
1.50 |
1.497 |
99.81 |
3.69 |
1.498 |
99.99 |
2.09 |
Stomach |
LLOQ |
0.25 |
0.248 |
99.15 |
0.90 |
0.250 |
99.97 |
1.61 |
|
LQC |
0.30 |
0.296 |
98.77 |
1.50 |
0.299 |
99.66 |
2.25 |
|
MQC |
0.70 |
0.698 |
99.69 |
0.91 |
0.701 |
100.11 |
0.93 |
|
HQC |
3.00 |
3.045 |
101.50 |
1.58 |
3.010 |
100.16 |
1.97 |
Accuracy (%), [(Obtained concentration/nominal concentration) x 100]; CV (%),
coefficient of variation [(S.D./mean) x 100]. |
