Biological Sample

Stomach

Blood

Large intestine

Small intestine

Mean ± SD^a

Accuracy^b

Mean ± SD^a

Accuracy^b

Mean ± SD^a

Accuracy^b

Mean ± SD^a

Accuracy^b

(µg/ml)

(%)

(µg/ml)

(%)

(µg/ml)

(%)

(µg/ml)

(%)

Low QC

Third freeze thaw

0.298 ± 0.006

99.21

0.304 ± 0.002

101.37

0.308 ± 0.010

102.79

0.699 ± 0.005

99.89

10 h (bench top)

0.302 ± 0.004

100.60

0.297 ± 0.006

99.14

0.308 ± 0.008

102.63

0.704 ± 0.004

100.55

24 h (in injector)

0.300 ± 0.005

99.94

0.300 ± 0.005

99.94

0.290 ± 0.007

96.57

0.678 ± 0.004

96.87

20 days at -80ºC

0.305 ± 0.003

101.63

0.295 ± 0.005

98.40

0.310 ± 0.005

103.40

0.695 ± 0.005

99.26

High QC

Third freeze thaw

3.214 ± 0.056

107.11

3.095 ± 0.052

103.15

3.070 ± 0.052

102.32

5.009 ± 0.047

100.17

10 h (bench top)

3.095 ± 0.113

103.15

3.046 ± 0.050

101.54

3.071 ± 0.063

102.36

5.084 ± 0.058

101.68

24 h (in injector)

2.938 ± 0.047

97.93

2.938 ± 0.047

97.93

3.089 ± 0.051

102.96

5.052 ± .056

101.03

20 days at -80ºC

2.969 ± 0.046

98.96

3.028 ± 0.054

100.92

3.113 ± 0.049

103.77

5.066 ± 0.062

101.31