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| Figure 3: iASPP-SV transformed NIH-3T3 cells. A. The stable clones of NIH 3T3 expressing FLAG-iASPP-SV and FLAG-iASPP (828) were established, and two randomly-selected stable clones were detected with FLAG antibody. B. Cell proliferation was assayed with cell counting kit-8 assay on d0, d2, d4 and d6. C. Representative plates from colony formation assay were shown. D. Numbers of colonies in soft agar per well were expressed as means and standard deviation. Two individual clones were randomly chosen, and each experiment was repeated three times in triplicate. |
