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Figure 1: Hemichrome formation associated with human HbO2 autoxidation, redrawn from Sugawara et al. [17]. UV/VIS spectral changes over time for hemichrome formation associated with human HbO2 autoxidation in which the reaction was monitored (a) in 0.1 M MES buffer (pH 5.0) at 37°C and scanning interval = 15 min; (b) in 0.1 M MES buffer (pH 6.5) at 40°C and scanning interval = 150 min; (c) in 0.1 M HEPES buffer (pH 8.0) at 40°C and scanning interval = 150 min; (d) in 0.1 M HEPES buffer (pH 7.0) at 45°C and scanning interval = 60 min. Conditions: HbO2 concentration was 235 μM (in heme contents).
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