The mean ± standard deviation of transit time of blood samples (s) for a given transit sample volume (μL)   Transit blood sample volume (μL) 20 μL 40 μL 60 μL Temperature-untreated reference 8.0 ± 1.08 16.3 ± 2.24 25.1 ± 3.50 Temperature-treated at 37 °C 7.8 ± 1.35 16.4 ± 2.57 26.4 ± 3.42 Temperature-treated at 42 °C 8.5 ± 1.38 ** 18.3 ± 2.94 ** 36.3 ± 6.32 ** Temperature-treated at 45 °C 9.3 ± 2.46 ** 21.2 ± 5.99 ** 41.8 ± 13.23 **

Table 2: Summary of the mean ± standard deviation of transit time of blood samples (s) for a given transit sample volume (μL),redrawn from Sugawara et al. [33]. Prior to application to MC-FAN, blood samples were subjected to mild heating at 37°C, 42°C and 45°C for 30 min, respectively. The number of subjects was 10. Significant differences (p<0.001) of the temperature-treated samples against the temperature-untreated reference were shown as **.

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