Case No Species H&E Fontana-Masson Bleaching method S-100  protein HMB-45 Tumor location Benign/ Malignant 1 Horse Melanocytoma + + + + Dermal Benign 2 Horse Melanocytoma + + + + Dermal Benign 3 Horse Melanocytoma + + ± ± Dermal Benign 4 Horse Melanocytoma + + ± ± Dermal Benign 5 Horse Melanocytoma + + + + Dermal Benign 6 Horse Melanoma + + ± ± ± Malignant 7 Cattle Melanocytoma + + + + Dermal Benign 8 Cattle Melanocytoma + + + + Dermal Benign 9 Cattle Melanocytoma + + + + Dermal Benign 10 Horse SCC - - - - - Malignant 11 Horse SCC - - - - - Malignant 12 Cattle SCC - - - - - Malignant 13 Cattle SCC - - - - - Malignant

Table 1: Comparative results of melanocytic tumors by H&E, special staining and Immunohistochemical methods.

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