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Figure 1: Effect of storage of blood in Cyto-Chex® BCT and K3EDTA tubes on viral load stability as determined by digital RT-PCR. Blood was drawn from each donor into three BCT and three K3EDTA tubes. Three tubes from each tube type were spiked with three different viral titers. A. K3EDTA and BCT blood were spiked with 4×104 viral copies/mL blood. B. K3EDTA and BCT blood were spiked with 4×105 viral copies/mL blood. C. K3EDTA and BCT blood were spiked with 4×106 viral copies/mL blood. Aliquots of blood were removed at indicated days, the plasma separated and M-PMV viral load or viral concentration was determined using digital RT-PCR as described in the “Materials and Methods” section. Error bars indicate SD. n=6.
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