Parameters Normal control Diabetic control  SAE treated (100mg/ kg BW) SAE treated (200mg/ kg BW) Glibenclamide treated (600μg/kg)
GSH (mM of DTNB conjugated/ mg protein)
Liver
Kidney
129.67 ± 2.66
118.77 ± 2.31
74.23 ± 1.51z
46.17 ± 2.49z
82.46 ± 2.27a
62.15± 3.56
98.12 ± 3.34c
69.38 ± 2.78
130.56 ± 1.41c
95.17 ± 2.55 (NS)
GST (m mol of CDNB-GSH conjugate formed/ min/ mg protein)
Liver
Kidney
6.75 ± 0.97
6.88 ± 0.34
3.42 ± 0.18 z
2.66 ± 0.27
3.56 ± 0.83b
3.09 ± 0.54a
3.98 ± 0.28c
3.68 ± 0.38b
5.12 ± 0.11b
3.76 ± 0.91b
GPx (mg glutathione consumed/ min/ mg protein)
Liver
Kidney
9.38 ± 0.91
7.33 ± 0.14
5.19 ± 0.88 z
4.99 ± 1.01y
5.82 ± 0.76b
5.08 ± 0.14a
6.42 ± 0.54c
5.92 ± 0.26c
7.24 ± 0.61b
5.66 ± 0.58a
CAT (mmol of H2O2 consumed/ min/ mg protein)
Liver
Kidney
71.25 ± 2.17
38.33 ± 1.22
38.67 ± 1.92 z
20.55 ± 1.75 z
44.32 ± 2.59b
21.26 ± 1.08a
58.45 ± 1.26c
28.52 ± 1.41c
65.31 ± 2.56c
35.24 ± 1.81c
SOD (U/ min/ mg/ Hb)
Erythrocytes 6.34 ± 0.19 3.41 ± 0.22 z 5.97 ± 0.62b 5.32 ± 0.43c 4.28 ± 0.24b
The values represent the means ± S. D. for six rats per group. ap<0.05, bp<0.01 and cp<0.001 compared to diabetic control group.yp<0.01, zp<0.001 as compared to normal; SOD: superoxide dismutase; GSH: Glutathione; GPx: glutathione peroxidase; GST: glutathione-S-transferase; CAT: Catalase.
Table 1: Effect of S. asoca extract on antioxidant enzyme activities in STZ-nicotinamide diabetic rats.
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