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| Figure 2: The comparison results for the linear dynamic range of Simple Western size assay and Western blot in anti-cancer kinase inhibitor screen cell model. The U2OS cell lysate was gradient diluted and analysed using pLLGL2-specific primary antibody. (A) Simple Western size assay lane view. The numbers (1&2) mean the samples were loaded in duplicate. The un-stimulated U2OS cell lysate was used as negative control. (B) Electropherogram and system software generated peak area and molecular weight data linear analysis (D). The traditional Western blot result (E) and bands intensity linear analysis (C, F). |
