#

Metabolite name

Class¹

Common Metabolic location

Average relative content2 ± standard deviation

p value

24 h

36 h

60 h

1

Lactate

OA

glycolysis pyruvate, propanoate metabolism

7.2 ± 0.3

7.2 ± 0.3

7.2 ± 0.5

0.0619

2

Glycolate

OA

glyoxylate, dicarboxylate metabolism

7.1 ± 0.1

6.7 ± 0.1

6.3 ± 0.1

0.0005

3

3-Hydroxy-propanoate

AA

beta-Alanine, propanoate metabolism

6.8 ± 0.2

6.9 ± 0.1

6.7 ± 0.2

0.4917

4

Leucine

AA

AA degradation/ synthesis ABC transporters aminoacyl-tRNAsynthesis

5.7 ± 0.1

5.0 ± 0.1

4.7 ± 0.3

0.0010

5

Malonate

OA

pyrimidine, beta-alanine metabolism

6.4 ± 0.3

5.9 ± 0.4

5.4 ±0.3

0.0274

6

Valine

AA

AA degradation / synthesis ABC transporters aminoacyl-tRNA biosynthesis propanoate metabolism pantothenate and CoA synthesis

5.3 ± 0.3

5.4 ± 0.2

5.1 ± 0.3

0.3959

7

Benzoate

OA

phenylalanine metabolism benzoate, biphenyl degradation alkaloid biosynthesis

5.7 ± 0.3

5.5 ± 0.2

5.3 ± 0.1

0.1454

8

Succinate

OA

TCA cycle oxidative phosphorylation AA metabolism glyoxylate, dicarboxylate, propanoate, butanoate metabolism

7.5 ± 0.1

6.4 ± 0.2

6.4 ± 0.1

0.0009

9

Dihydroxy-butanoate

OA

butanoate metabolism

7.1 ± 0.2

6.7 ± 0.1

6.2 ± 0.1

0.0002

10

Erythrose

S

carbohydrate metabolism

6.2 ± 0.3

6.3 ± 0.1

5.5 ± 0.4

0.0248

11

Proline

AA

urea cycle AA metabolism aminoacyl-tRNA synthesis ABC transporters

6.7 ± 0.1

6.8 ± 0.1

6.3 ± 0.3

0.0491

12

Threonate

OA

ascorbate and aldarate

6.4 ± 0.2

6.3 ± 0.2

6.3 ± 0.3

0.5087

13

Glycerol

SA

galactose, glycerolipid metabolism

7.2 ± 0.2

6.8 ± 0.1

6.3 ± 0.4

0.0137

14

Glutamine

AA

purine, pyrimidine, glutamate, nitrogen metabolism aminoacyl-tRNA biosynthesis ABC transporters

7.6 ± 0.7

8.0 ± 0.1

7.5 ± 0.3

0.4411

15

Scyllo-inosose

S

inositol/ inositol phosphate metabolism

6.3 ± 0.6

5.3 ± 0.7

5.7 ± 0.4

0.1310

16

Threonic acid

OA

n/a3

6.4 ± 0.1

6.2 ± 0.1

6.1 ± 0.2

0.0576

17

Glycerol-3-phosphate

PSA

glycerolipid, glycerophospholipid metabolism ABC transporters

6.9 ± 0.1

6.3 ± 0.2

6.5 ± 0.5

0.1878

18

Citrate

OA

TCA cycle AA, glycoxylate, dicarboxylate metabolism reductive carboxylate cycle

6.4 ± 0.6

5.9 ± 0.4

5.8 ± 0.4

0.3533

19

Inositol*

SA

inositol, galactose, inositol phosphate metabolism phosphatidylinositol signaling system

8.6 ± 0.0

8.3 ± 0.1

8.0 ± 0.2

0.0034

20

3 Deoxy-hexonate

OA

n/a³

7.9 ± 0.1

7.6 ± 0.1

7.3 ± 0.2

0.0032

21

Fructose*

S

fructose, mannose, galactose, starch and sucrose metabolism

8.2 ± 0.0

8.0 ± 0.0

7.7 ± 0.1

0.0005

22

Fructose†

S

fructose, mannose, galactose, starch and sucrose metabolism

8.1 ± 0.0

7.9 ± 0.0

7.6 ± 0.3

0.0004

23

Galactose

S

galactose metabolism ABC transporters bacterial chemotaxis

6.5 ± 0.1

6.0 ± 0.2

5.6 ± 0.3

0.0109

24

Inositol†

SA

inositol, galactose, inositol phosphate metabolism phosphatidylinositol signaling

7.2 ± 0.4

6.9 ± 0.2

6.1 ± 0.3

0.0081