A: Adherence and proliferation of BMMSC.
B: Morphological changes during BMMSC osteocytic differentiation at day 10 following treatment with dexamethasone or Colloss E on Hematoxyllin and Eosin stained cover slips.
C: ALP protein expression in treated versus untreated BMMSCs at day 8 post treatment. GAPDH was used to ensure equal loading of cell lysates.
D: Expression of alkaline phosphatase activity in U/ml/hour/μg of protein. The peak was detected at day 10 post-treatment with Dexamethasone and Colloss
E. A statistically significant increase in ALP activity * was detected in treated versus non treated cells.
