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| Figure 2: Morphology and stem cell marker staining of center cut and non-cut colonies. Left: Phase contrast images showing the result of surgically removing the central part of the colony on Day 4 and control colony was undisturbed. Right: Confocal images of colonies stained with the nuclear dye Hoechst 33342 and fluorescently labeled antibodies against OCT-4 at Days 3 and 7 after cutting. Scale bar represents 100 µm. |
