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| Figure 4: hESC attachment and colony formation on inserts/glass cover-slip pre-coated with MEF. Floating cells in cultures using inserts with MEF formed colonies but did not form in cultures with glass cover slip. Although there were few cells attached to glass surface, they were not pluripotent based on weak staining for OCT-4 and SSEA-4. Scale bar represents 100 µm. |
