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Figure 5: Cell viability levels in cell-scaffold cultures. Cell viability was measured using MTS-based proliferation assay at days 1, 6, and 14.
a) The number of viable wild type (wt) cells in culture remained at 95% or greater of the 125,000 total cells initially embedded within NF and NF+MMP scaffolds. This was significantly higher than Matrigel cultures, where the number of viable cells by day 14 was less than 70% of initial cells numbers.
b) The number of viable diabetic (db) cells in culture remained at 95% or greater of the 125,000 total cells initially embedded within NF and NF+MMP scaffolds. This was significantly higher than Matrigel cultures, where the number of viable cells by day 14 was at 82% of initial cells numbers. All data were normalized to day 1 values for analyses. * p<0.05 when compared to NF-based samples, ^ p <0.05 when compared to day 1 samples of same experimental group, # p<0.05 when compared to wt samples of same experimental groups.
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