| Research Article |
Open Access |
|
| Determination of Doxycycline in Human Plasma by Liquid
Chromatography-Mass Spectrometry after Liquid-Liquid
Extraction and its Application in Human Pharmacokinetics
Studies |
| Muralidharan Selvadurai, Subramania Nainar Meyyanathan*, Sekar Rajan, Govindarajan Padmanaban and Bhojraj Suresh |
| Department of Pharmaceutical Analysis, J.S.S.College of pharmacy, Ootacamund 643001, Tamilnadu, India |
| *Corresponding author: |
Dr. Subramania Nainar Meyyanathan Ph.D
Professor
and Head
Department of pharmaceutical Analysis
J.S.S.College of pharmacy
Ootacamund-643001
Tamilnadu, India
E-mail: meyys@rediffmail.com |
|
| |
| Received July 15, 2010; Accepted September 16, 2010; Published September
16, 2010 |
| |
| Citation: Selvadurai M, Meyyanathan SN, Rajan S, Padmanaban G, Suresh B (2010) Determination of Doxycycline in Human Plasma by Liquid Chromatography-
Mass Spectrometry after Liquid-Liquid Extraction and its Application in Human
Pharmacokinetics Studies. J Bioequiv Availab 2: 093-097. doi:10.4172/jbb.1000038 |
| |
| Copyright: © 2010 Selvadurai M, et al. This is an open-access article distributed
under the terms of the Creative Commons Attribution License, which permits
unrestricted use, distribution, and reproduction in any medium, provided the
original author and source are credited. |
| |
| Abstract |
| A sensitive and reproducible liquid chromatography–mass spectrometric (LC-MS) method has been developed and
validated for the quantifi cation of doxycycline in human plasma, after liquid-liquid extraction (LLE). Best chromatographic
resolution was achieved on a reverse-phase Phenomenex C18 column using the mobile phase acetonitrile–5mM
ammonium acetate (80:20) in isocratic elution with a total run time of 3.5 min. The analyte, doxycycline was detected
by using an electrospray ionization mass spectrometer in the selected ion monitoring (SIM) mode. Linear plot was
obtained in the concentration range of 0.5–5.0 μg/ml (r2 = 0.998). Lower limit of quantifi cation (LLOQ) was found to be
100 ng/ml in 500μl of plasma. Average recovery of the analyte was found to range from 96.08 to 104.60% in plasma at
the concentrations of 1.0, 3.0 and 5.0 μg/ml. Intra- and inter-day relative standard deviation was found to be 0.13-0.74
and 0.11-0.34 respectively. The present method was successfully applied in the pharmacokinetic study of doxycycline
in human plasma. |
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