Different extraction techniques |
Observed phospholipids |
MF
(%CV)¥ |
Retention window of nevirapin |
Complete analysis time# |
Known phospholipids responsible for ME≠ |
Pre-cursor ion scanning at |
m/z 104 |
m/z 184 |
m/z 104 |
m/z 184 |
m/z 184 |
Methanol precipitation |
Nil |
497.4, 523.4, 759.9, 785.6 and 811.7 |
497.6, 521.5, 523.5, 525.7, 543.6, 545.5, 548.0 and 567.3 |
497.5, 521.4, 759.8, 787.5 and 811.7 |
704 (PC),
758 (PC) and
786 (PC). |
0.30
(%CV 46.0) |
Acetonitrile precipitation |
Nil |
759.7, 760.8, 783.6, 785.6, 787.6 and 811.8 |
497.8, 521.6, 523.8, 525.7, 543.6, 545.6, 547.6, 564.0, and 567.6 |
59.8, 785.6 and 811.7 |
758 (PC) and
786 (PC). |
0.26
(%CV 41.47) |
Liquid-liquid extraction with ethyl acetate |
Nil |
759.7, 783.6, 785.6, 788.2, 811.6 and 815.2 |
497.6, 521.6, 523.5, 525.6, 543.7, 545.7, 547.6, and 568.3. |
497.4, 521.3,
523.4, 787.6,
790.5 and 811.8 |
758 (PC) and
786 (PC). |
0.79
(%CV 6.68) |
Liquid-liquid extraction with methyl tertiary butyl ether |
Nil |
Nil |
Nil |
759.8, 760.6, 786.3, 787.5, and 811.7 |
758 (PC) and
786 (PC). |
0.80
(%CV 6.14) |
Solid phase extraction (Merck, Lichrosep catridge) |
Nil |
759.9, 787.7 and 811.7 |
497.8, 521.8, 543.3, and 545.5 |
759.8, 787.5, and 811.8 |
Nil |
0.98
(%CV 1.44) |
Solid phase extraction (Waters, Oasis HLB catridge) |
Nil |
759.7, 783.5, 787.5 and 811.7 |
497.5, 521.6, 523.6, and 543.9 |
759.8, 785.8 and 811.6 |
Nil |
0.99
(%CV 1.92) |
|
