Different extraction techniques

Observed phospholipids

MF
(%CV)¥

Retention window of nevirapin

Complete analysis time#

Known phospholipids responsible for ME≠

Pre-cursor ion scanning at

m/z 104

m/z 184

m/z 104

m/z 184

m/z 184

Methanol precipitation

Nil

497.4, 523.4, 759.9, 785.6 and 811.7

497.6, 521.5, 523.5, 525.7, 543.6, 545.5, 548.0 and 567.3

497.5, 521.4, 759.8, 787.5 and 811.7

704 (PC),
758 (PC) and
786 (PC).

0.30
(%CV 46.0)

Acetonitrile precipitation

Nil

759.7, 760.8, 783.6, 785.6, 787.6 and 811.8

497.8, 521.6, 523.8, 525.7, 543.6, 545.6, 547.6, 564.0, and 567.6

59.8, 785.6 and 811.7

758 (PC) and
786 (PC).

0.26
(%CV 41.47)

Liquid-liquid extraction with ethyl acetate

Nil

759.7, 783.6, 785.6, 788.2, 811.6 and 815.2

497.6, 521.6, 523.5, 525.6, 543.7, 545.7, 547.6, and 568.3.

497.4, 521.3,
523.4, 787.6,
790.5 and 811.8

758 (PC) and
786 (PC).

0.79
(%CV 6.68)

Liquid-liquid extraction with methyl tertiary butyl ether

Nil

Nil

Nil

759.8, 760.6, 786.3, 787.5, and 811.7

758 (PC) and
786 (PC).

0.80
(%CV 6.14)

Solid phase extraction (Merck, Lichrosep catridge)

Nil

759.9, 787.7 and 811.7

497.8, 521.8, 543.3, and 545.5

759.8,  787.5, and 811.8

Nil

0.98
(%CV 1.44)

Solid phase extraction (Waters, Oasis HLB catridge)

Nil

759.7, 783.5, 787.5 and 811.7

497.5, 521.6, 523.6, and 543.9

759.8, 785.8 and 811.6

Nil

0.99
(%CV 1.92)

Table 1: Observed phospholipids during different extraction techniques.
Goto home»