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| Figure 2: Proposed model of the events taking place in the CGTase-catalyzed reactions. (1) Disproportionation, (2) Coupling, (3) Cyclization. The different CGTase domains are indicated (A, B, C, D, and E). 1 and 2 indicate the maltose binding sites on the E-domain. The triangle indicates the cleavage site in the active site. Circles represent glucose residues; acceptor residues are represented in black. Reprinted from [23].The three transglycosylation reactions catalyzed by cyclodextrin glycosyltransferase from Bacillus circulans (strain 251) proceed via different kinetic mechanisms, 267 pp 658-665, Reprinted with permission. Copyright (2000) FEBS. |
