| a) |
| Healthy |
Tissue hydration (water content g/g dry mass) |
| Organ |
Ouabain concentration (M) |
|
|
|
|
|
| 0 |
10-11 |
Δ% |
Ouabain
10-8M |
Δ% |
Ouabain
10-6M |
Δ% |
| Heart muscle |
2.389±0.1 |
2.422±0.02 |
↑1.38 |
2.7±0.26 |
↑13.02 |
2.306±0.05 |
↓3.47 |
| Skeletal muscle |
2.357±0.23 |
2.338±0.12 |
↓0.8 |
3.361±0.25 |
↑42.6 |
3.421±0.3 |
↑45.14 |
| Lung |
2.199±0.15 |
2.385±0.15 |
↑8.4 |
2.243±0.06 |
↑2.01 |
2.167±0.3 |
↓1.46 |
| Liver |
2.054±0.12 |
2.337±0.22 |
↑13.78 |
2.547±0.18 |
↑24.01 |
2.92±0.17 |
↑42.16 |
| Spleen |
2.225±0.13 |
1.567±0.08 |
↓29.57 |
2.444±0.25 |
↑9.84 |
3.147±0.3 |
↑41.44 |
| Kidney |
2.05±0.13 |
1.722±0.08 |
↓16.04 |
2.878±0.19 |
↑40.32 |
3.09±0.15 |
↑50.66 |
|
| b) |
| Sarcoma carrying |
Tissue hydration (water content g/g dry mass) |
| Organ |
Ouabain concentration (M) |
|
|
|
|
|
| 0 |
10-11 |
Δ% |
Ouabain 10-8M |
Δ% |
Ouabain
10-6M |
Δ% |
| Heart muscle |
3.3±0.1 |
3.25±0.01 |
↓1.5 |
2.5±0.01 |
↓24.24 |
3±0.01 |
↓9.09 |
| Skeletal muscle |
3.87±0.21 |
2.64±0.14 |
↓31.78 |
4.63±0.33 |
↑19.64 |
4.41±0.23 |
↑13.95 |
| Lung |
3.31±0.03 |
3.62±0.21 |
↑9.37 |
5.75±0.18 |
↑73.72 |
3.67±0.23 |
↑10.88 |
| Liver |
3.265±0.15 |
2.95±2.27 |
↓9.64 |
3.88±0.35 |
↑18.84 |
3.94±0.22 |
↑20.67 |
| Spleen |
2.8±0.14 |
2.7±0.14 |
↓3.57 |
2.93±0.33 |
↑4.64 |
3.14±0.23 |
↑12.14 |
| Kidney |
3.357±0.19 |
3.52±0.16 |
↑4.86 |
3.56±0.16 |
↑6.05 |
3.44±0.16 |
↑20.47 |
| Sarcoma 180 |
4.8±0.33 |
4.4±0.4 |
↓8.33 |
5.66±0.28 |
↑17.92 |
4.97±0.32 |
↑3.54 |
|
| Table 2: The effect of ouabain (at 10-11, 10-8 and 10-6M concentrations) on tissue hydration of different organs: a) healthy mice;
b) sarcoma-180 carrying mice. |
