| a) |
| Organ |
Ouabain concentration (M) |
| 0 |
10-11 |
10-8 |
10-6 |
control |
cisPt |
Δ% |
control |
cisPt |
Δ% |
control |
cisPt |
Δ% |
control |
cisPt |
Δ% |
| Heart muscle |
2.39 ±0.1 |
1.93 ±0.15 |
↓19.07 |
2.42 ±0.02 |
1.38 ±0.12 |
↓42.89 |
2.7 ±0.26 |
1.99 ±0.13 |
↓26.1 |
2.3 ±0.05 |
2.47 ±0.19 |
↑7.3 |
| Skeletal muscle |
2.36 ±0.23 |
3.08 ±0.21 |
↑30.47 |
2.34 ±0.06 |
2.76 ±0.12 |
↑18.23 |
3.36 ±0.25 |
3.25 ±0.29 |
↓3.2 |
3.42 ±0.3 |
3.76 ±0.27 |
↑9.95 |
| Lung |
2.2 ±0.15 |
1.21 ±0.11 |
↓45.06 |
2.39 ±0.15 |
0.88 ±0.11 |
↓63.17 |
2.24 ±0.06 |
1.11 ±0.12 |
↓50.5 |
2.17 ±0.17 |
1.69 ±0.16 |
↓22.15 |
| Liver |
2.05 ±0.12 |
2.27 ±0.12 |
↑10.24 |
2.34 ±0.22 |
2.32 ±0.19 |
↓0.74 |
2.55 ±0.18 |
2.98 ±0.2 |
↑17.2 |
2.92 ±0.17 |
2.43 ±0.17 |
↓16.94 |
| Spleen |
2.23 ±0.13 |
2.13 ±0.12 |
↓4.12 |
1.57 ±0.17 |
1.6 ±0.15 |
↑2.13 |
2.44 ±0.13 |
1.55 ±0.07 |
↓36.6 |
3.15 ±0.26 |
1.75 ±0.14 |
↓44.4 |
| Kidney |
2.05 ±0.13 |
2.05 ±0.12 |
↓0.12 |
1.72 ±0.08 |
2.41 ±0.13 |
↑39.85 |
2.88 ±0.19 |
2.46 ±0.18 |
↓14.6 |
3.09 ±0.15 |
2.67 ±0.19 |
↓13.73 |
|
| b) |
| Organ |
Ouabain concentration (M) |
| 0 |
10-11 |
10-8 |
10-6 |
| control |
cisPt |
Δ% |
control |
cisPt |
Δ% |
control |
cisPt |
Δ% |
control |
cisPt |
Δ% |
| Heart muscle |
3.3 ±0.1 |
3.34 ±0.08 |
↑1.3 |
3.25 ±0.1 |
2.55 ±0.07 |
↓21.54 |
2.5 ±0.4 |
2.88 ±0.15 |
↑15 |
3 ±0.12 |
3.05 ±0.18 |
↑1.67 |
| Skeletal muscle |
3.87 ±0.21 |
4.07 ±0.18 |
↓30.91 |
3.53 ±0.14 |
4.78 ±0.16 |
↑15.37 |
4.62 ±0.33 |
4.74 ±0.16 |
↓6.72 |
4.41 ±0.23 |
4.81 ±0.31 |
↑1.46 |
| Lung |
3.31 ±0.03 |
2,83 ±0.13 |
↓14.39 |
2.64 ±0.21 |
3.1 ±0.14 |
↑17.54 |
5.75 ±0.18 |
3.25 ±0.15 |
↓43.48 |
3.67 ±0.15 |
2.5 ±0.19 |
↓31.81 |
| Liver |
3.27 ±0.15 |
2.4 ±0.17 |
↓26.43 |
3.62 ±0.27 |
2.98 ±0.1 |
↓17.58 |
3.88 ±0.35 |
3.58 ±0.2 |
↓7.76 |
3.94 ±0.15 |
3.92 ±0.12 |
↓0.55 |
| Spleen |
2.8 ±0.14 |
2.42 ±0.12 |
↓13.69 |
2.95 ±0.06 |
1.88 ±0.19 |
↓36.54 |
2.93 ±0.13 |
2.33 ±0.01 |
↓20.33 |
3.14 ±0.19 |
2.86 ±0.2 |
↓8.98 |
| Kidney |
3.36 ±0.19 |
2.25 ±0.2 |
↓32.98 |
2.69 ±0.16 |
2.85 ±0.25 |
↑5.81 |
3.56 ±0.16 |
3.17 ±0.24 |
↓10.94 |
3.44 ±0.15 |
2.88 ±0.18 |
↓16.36 |
| Sarcoma-180 |
4.8 ±0.33 |
4.08 ±0.35 |
↓15.16 |
4.41 ±0.4 |
4.78 ±0.15 |
↓8.37 |
5.66 ±0.23 |
4.74 ±0.23 |
↓16.25 |
4.98 ±0.32 |
4.81 ±0.36 |
↓3.25 |
|
| Table 4: The effect of cisPt on tissue hydration of different organs: a) healthy mice; b) sarcoma-180 carrying mice. |
