Ouabain content (Mol)                                     Incubation medium
Hypotonic Isotonic Hypertonic
1x10-10 4.59 ± 0.32 3.23 ± .24 2.03 ± 0.16
3x10-10 18.3 ± 1.4 11.7 ± .87 6.29 ± 0.41
6x10-10 28.9 ± 2.0 17.9 ± 1.2 10.0 ± 0.67
1x10-9 32.0 ± 2.2 21.1 ± 1.4 12.2 ± 0.9
3x10-9 144 ± 29.4 90.5 ± 5.7 53.8 ± 3.1
6x10-9 431 ± 29.4 266 ± 15.8 147 ± 9.7
1x10-8 793 ± 45.6 508 ± 30.1 283 ± 19.4
Table 1: Binding of [3H] Ouabain to Helix pomatia Cell Membrane as a Function of Concentration of Glycoside in Solutions with Different Tonicity ( ×108 molecules/mg dry weight).
Goto home»