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| Figure 2: Study of the bio-production of SCFAs by L. reuteri strains in cell culture conditioned medium (CM): In order to establish if L. reuteri NCIMB -11951, -701089, -701359, -702655, and -702656, produces SCFAs known as active anti-cancer compounds in-vitro conditions; the active bacterial cells were incubated in DMEM (2 h, 37°C, 5% CO2). Then, the acetic, propionic, and butyric acids were separated and quantified by HPLC method. L. acidophilus ATCC 314 is used as a control. Data are presented as mean ± SEM (n = 3). *p < 0.05, **p < 0.01 and ***p < 0.001, compared with L. reuteri NCIMB - 701089. |
