|
| Figure 3: Screening of L. reuteri strains for a potential anti-proliferative effect against colon cancer cells. To investigate the anti-proliferative effect of probiotic L. reuteri bacteria, the cell culture conditioned medium (CM) of L. reuteri NCIMB -11951, -701089, -701359, -702655, and -702656 was used. The viability and growth inhibition of human epithelial CRC adenocarcinoma cells (Caco-2) by the L. reuteri-CM was measured after incubation with probiotic treatments for (a) 24 h, (b) 48 h and (c) 72 h, using ATP bioluminescence. L. acidophilus ATCC 314 is used as a positive control for comparative purposes. Data are presented as mean ±SEM (n=4). *p<0.05, * p < 0.01 and ***p<0.001, compared with L. reuteri NCIMB 702655. |
