|
| Figure 4: Correlation analysis between the production of probiotic SCFAs and the inhibition of colon cancer cell by probiotic bacteria L. reuteri. The correlation was determined between the concentrations of (a) acetate, (b) propionate, (c) butyrate and (d) total SCFAs in L. reuteri-CM and the inhibition of colon cancer cell growth by L. reuteri-CM. This test was performed to examine if L. reuteri-CM suppressed colon cancer cell growth due, in part, to the presence of SCFAs produced by the probiotic bacteria. The inhibition of Caco-2 proliferation by L. reuteri-CM was measured after 72 h of treatment. SCFAs were measured in the L. reuteri-CM used to treat Caco-2 cells. Plots represent the data of cell growth inhibition described in Fig. 2. The lines were obtained by linear regression analysis. |
