Corresponding CM SSF Composition (mg/L) SSF+LA Composition (mg/L)     Acetate Propionate Butyrate   Lactate Acetate Propionate Butyrate L. a314 SSF-a 114 0 14 SSF-a+LA 1948 114 0 14 L. r 11951 SSF-r1 131 111 29 SSF-r1+LA 369 131 111 29 L. r 701089 SSF-r8 43 38 0 SSF-r8+LA 208 43 38 0 L. r701359 SSF-r13 116 78 28 SSF-r13+LA 643 116 78 28 L. r 702655 SSF-r55 86 45 29 SSF-r55+LA 233 86 45 29 L. r 702656 SSF-r56 182 161 59 SSF-r56+LA 642 182 161 59

Table 1:Composition of SCFA synthetic formulations (SSFs) containing different concentrations of acetate, propionate and butyrate (no bacteria were used), designed at the respective concentrations of naturally produced SCFAs in the CM of L. reuteri NCIMB -11951, -701089, -701359, -702655, and -702656. Another set of SSF was prepared by the addition of respective concentrations of lactate same as the one produced by L. reuteri-CM. SSF: SCFA synthetic formulation.

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