Mobile phase Composition
A 50% methanol, 50% phosphate buffer pH 7.4;
B 50% acetonitrile, 50% Milli-Q water
C 20% acetonitrile, 80%,20 mM phosphate buffer pH 7.4
D 20%methanol,80% 1% acetic acid bufferpH3.0;
E 65% methanol,35% 0.7% phosphate buffer pH 3.5
F 20% acetonitrile, 80% Milli-Q water
G 3% phosphate buffer pH 7.4 20 mM, 24% Milli-Q water, 40% acetonitrile, 33% methanol
H 15% methanol in Milli-Q watercontaining 0.005MHCland 0.5Msodium chloridepH3.0
I 55% methanol, 45% phosphate buffer pH 5.2
J 75% methanol, 25% Milli-Q water;
K 55% methanol,1% acetic acid buffer pH3.0
L 55% methanol,45% phosphate buffer pH 5.2;
Table 2: aMobile phase Composition.
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