Mobile phase |
Composition |
A |
50% methanol, 50% phosphate buffer pH 7.4; |
B |
50% acetonitrile, 50% Milli-Q water |
C |
20% acetonitrile, 80%,20 mM phosphate buffer pH 7.4 |
D |
20%methanol,80% 1% acetic acid bufferpH3.0; |
E |
65% methanol,35% 0.7% phosphate buffer pH 3.5 |
F |
20% acetonitrile, 80% Milli-Q water |
G |
3% phosphate buffer pH 7.4 20 mM, 24% Milli-Q water, 40% acetonitrile, 33% methanol |
H |
15% methanol in Milli-Q watercontaining 0.005MHCland 0.5Msodium chloridepH3.0 |
I |
55% methanol, 45% phosphate buffer pH 5.2 |
J |
75% methanol, 25% Milli-Q water; |
K |
55% methanol,1% acetic acid buffer pH3.0 |
L |
55% methanol,45% phosphate buffer pH 5.2; |