Sample Official method Standard addition method
  Pure solutions   Ag Cu G GC Ns-ST/Ag (FIA)
X ± S.E. 99.8 ± 0.2 99.7 ± 0.5 98.5 ± 0.5 99.2 ± 0.5 99.6 ± 0.6 99.2 ± 0.5
F value   1.75 2.61 2.15 2.45 2.55
t value   2.47 2.33 1.84 2.22 1.65
Amostigmine tablets (15 mg/tablet)        
X ± S.E. 101.3 ± 0.2   101.0 ± 0.5 102.0 ± 0.5 101.5 ± 0.5 101.0 ± 0.5 102.5 ± 0.5
F value   2.42 2.33 2.65 2.18 2.56
t value   1.36 2.16 1.46 1.69 1.35
Amostigmine injection (0.5 mg/mL)        
X ± S.E. 99.6 ± 0.07   99.5 ± 0.5 98.5 ± 0.5 99.5 ± 0.5 99.5 ± 0.5 101.5 ± 0.5
F value   2.76 2.78 2.45 2.55 2.45
t value   1.86 1.97 1.87 1.37 1.22
Spiked Urine              
X ± S.E. 99.6 ± 0.07   99.0 ± 0.5 98.0 ± 0.5 99.0 ± 0.5 99.5 ± 0.5 102.0 ± 0.5
F value     2.73 2.62 2.92 4.11 2.61
t value     1.87 1.54 2.11 3.27 1.77
Sample Official method Potentiometric titration
Pure solutions Ag Cu G GC Ns-ST/Ag (FIA)
X ± S.E. 99.8 ± 0.2   99.5 ± 0.5 98.5 ± 0.5 99.5 ± 0.5 99.5 ± 0.5 100.5 ± 0.5
      2.42 2.22 2.26 2.33 2.04
      1.52 1.14 1.56 1.52 1.93
Amostigmine tablets (15 mg/tablet)
X ± S.E. 100.3 ± 0.2   101.5 ± 0.5 102.0 ± 0.5 101.5 ± 0.5 101.5 ± 0.5 101.5 ± 0.09
      2.54 2.96 2.56 2.41 2.33
      1.84 1.44 1.87 1.85 1.70
Amostigmine injection (0.5 mg/mL)
X ± S.E. 99.8 ± 0.06   100.5 ± 0.5 101.5 ± 0.5 101.0 ± 0.5 101.5 ± 0.5 101.5 ± 0.5
F value     2.90 2.88 2.48 2.34 2.71
t value     1.25 1.96 1.66 1.15 1.09
X ± S.E.: Recovery ± standard error.
F- tabulated is 6.39 at 95.0% confidence limit.
t- tabulated at 99.0% confidence limit and 6 degrees of freedom
Table 7: Statistical treatment of data obtained for the determination of neostigmine bromide applying the standard addition method and potentiometric titration using CW sensors in comparison with official methods.
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