| Sample |
Protein concentration (µg/ml plasma) |
TT (sec) |
%TT |
| Control N® |
|
17.10 ± 1.14 |
|
| Citrated plasma |
-- |
18.30 ± 0.61 |
0 |
| LSE1 |
12.393 |
28.37 ± 6.66 |
55.01 ± 3.59α |
| 18.589 |
38.83 ± 7.5 |
112.20 ± 4.11α |
| 21.688 |
53.03 ± 0.59 |
189.80 ± 3.24α |
| 24.786 |
64.70 ± 1.36 |
253.55 ± 7.44α |
| Citrated plasma |
-- |
17.43 ± 1.01 |
0 |
| LSE2 |
11.969 |
26.50 ± 0.46 |
52.01 ± 2.65α |
| 23.938 |
34.50 ± 1.21 |
97.90 ± 6.92α |
| 29.9227 |
41.23 ± 2.01 |
136.55 ± 6.67α |
| 35.907 |
47.17 ± 1.01 |
170.55 ± 3.33α |
| Citrated plasma |
-- |
22.70 ± 0.13 |
0 |
| LSE3 |
12.536 |
23.50 ± 0.50 |
3.52 ± 2.22 |
| 18.804 |
29.43 ± 0.79 |
29.66 ± 3.46β |
| 25.072 |
36.97 ± 1.07 |
62.85 ± 4.73α |
| 31.341 |
47.03 ± 1.62 |
107.20 ± 7.15α |
| Citrated plasma |
-- |
17.1 ± 1.14 |
0 |
| LSE4 |
24.164 |
22.43 ± 1.11 |
31.19 ± 6.49β |
| 40.274 |
30.57 ± 1.58 |
78.75 ± 9.22α |
| 48.329 |
35.17 ± 0.52 |
105.65 ± 3.04α |
| 56.384 |
41.43 ± 0.79 |
142.30 ± 4.60α |
LSE1: leech saliva extract collected from starved leeches in May
(sunny season).
LSE2: leech saliva extract collected from starved leeches starved in November
(rainy season).
LSE3: leech saliva extract collected from starved leeches in
December
(rainy season).
LSE4: leech saliva extract collected from starved
leeches in January
(rainy season).
TT: thrombin time determined using thrombin
time assay in vitro. %
TT: the percentage increase in thrombin time. All results are
expressed as the mean of triplicates ± SEM and analyzed by One-Way ANOVA
using SPSS 18.0 software.
αρ<0.001 when compared with %TT of the citrated plasma (zero point).
βρ<0.05
when compared with %TT of the citrated plasma (zero point). |
