| LC-MS/MS parameter |
Description |
| HPLC |
Agilent 1200 |
| Software Version |
Mass Hunter Version B.01.04 |
| Sample Preparation |
Plasma Protein precipitation |
| Sample Matrix |
Plasma + buffer |
| Sample Volume |
100 µL |
| Column |
X Bridge C18 5 µM (4.6 Χ 50 mm) |
| Mobile Phase |
(a) 0.1% formic acid in Water |
| |
(b) 0.1% formic acid in Acetonitrile |
| Flow rate (mL/min) |
0.8 mL/min |
| Column Temperature (°C) |
25 |
| Injection Volume (µL) |
10 |
| Run time (min) |
20 |
| Flow |
Gradient |
| QQQ Mass Spectrometer parameter |
Description |
Ion Source
Polarity
Delta EMV
Start mass
End mass
Scan mode
Scan time (Dwell)
Fragmentation Voltage
Gas Temperature (°C)
Nebulizer (PSI)
Capillary Voltage
Chamber Current (μA)
Capillary Current (nA)
MS1 Heater (°C)
MS2 Heater (°C)
Turbo1 Speed (%)
Turbo 1 Power (watt) |
ESI (Electrospray)
Positive
0
100
1000
EIC (Extracted Ion Chromatogram)
500
135
300
45
4000
0.34
13
100
100
100
104.2 |
