Intra-batch Validation (n=7)

Nominal concentration (ng/mL)

1

3

30

400

Mean (ng/mL)

0.95

3.17

31.74

423.57

SD (ng/mL)

0.04

0.15

0.66

4.79

Precision (%)

4.49%

4.77%

2.08%

1.13%

Accuracy (%)

94.79%

105.57%

105.81%

105.89%

Inter-batch Validation (n=21)

Nominal concentration (ng/mL)

1

3

30

400

Mean (ng/mL)

0.96

3.17

31.29

421.24

SD (ng/mL)

0.05

0.11

0.68

5.00

Precision (%)

5.03%

3.41%

2.16%

1.19%

Accuracy (%)

96.23%

105.79%

104.29%

105.31%