|
| Figure 3: Treatment of BMSC and the cell lines KGI a and K562 with the LSL fucoidan (Fu) resulted in rapid externalization of the chemokine receptor CXCR4, as measured by immunostaining and now cytometry. Row A shows the baseline expression of CXCR4 on UT cells. Row B shows the level of CXCR4 expression after treatment with Fu. Row C shows the inhibition of CXCR4 expression on KG Ia and CD34+ BMSC cells where both Fu and AFA-W were added simultaneously. Row C also shows that the effect did not extend to the CD34- cell line K562. The last row D shows that AFA-W alone did not trigger CXCR4 expression on the cell surface. The data shown are representative of testing involving three different bone marrow samples and three separate experiments involving the two cell lines. |
