Expansion of cytotoxic T lymphocytes

Donor	tetramer staining (%)		cytotoxicity (%)
Donor	HLA-A2/pHIV	HLA-A2/ pSurvivin_95-104	T2/ p Survivin_95-104	U251	T2pHIV
1	0.56	94.2	72.8	70.9	0.7
2	0.43	96.4	79.7	74.9	1.4
3	0.67	97.9	80.1	72.9	1.6
4	0.73	92.1	74.3	73.7	1.3
5	0.62	95.8	76.2	75.1	1.2

Table 1: Expansion of cytotoxic T lymphocytes (CTLs) specific for pSurvivin_95-104 in five human leukocyte antigen (HLA)-A2 positive healthy donors. The CTLs expanded by the W6/32 antibody-mediated pSurvivin_95-104-specific aAPCs were tested for tetramer staining and cytotoxic activity. Tetramer staining cells indicate the percentage of viable CD8⁺ HLA-A2/pSurvivin_95-104 tetramer-positive or CD8⁺ HLA-A2/pHIV tetramer-positive lymphocytes in the CD8+ population of viable lymphocytes; lysis indicates the percent lysis of pSurvivin_95-104-pulsed T2 cells (T2pSurvivin_95-104), U251 (HLA-A2⁺, Survivin⁺) or pHIV-pulsed T2 cells (T2pHIV). Results are shown for an effector / target ratio of 8:1.

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