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| Figure 3: Analysis of the SCCs at different ages of the prepared larvae [ATU – accumulated thermal unit, day degree]. (a) Formation ratio as the relation number of developed SCC and prepared larvae (18 larvae at each preparation time) and the relation of larvae which developed 1 to 5 SCC. The distribution exhibits normality (p < 0.05; Kolmogorov-Smirnov test). (b) Formation ratio of SCCs in relation to the time. (c) Box-Whisker-plot of the size of the SCCs in dependence of the age of the prepared larvae. Illustrated are minimum and maximum (crossbars at the end), median (grey bar) and upper and lower quartile (borders of the bar). Significant differences between the group of 252 ATU and 294 ATU (p < 0.05, Kruskal-Wallis-signed-rank-test). The size of the SCCs rises significantly with the age of the larvae (p < 0.05, Wilcoxon signed-rank-test). (d) Box-Whisker-plot of the contraction frequency of the SCCs in dependence of the age of the prepared larvae. Illustrated are minimum and maximum (crossbars at the end), median (grey bar) und upper and lower quartile (borders of the bar). Significant differences between the groups (p < 0.05, Kruskal-Wallis-signed-rank-test) exist. The beating frequency was lower at 252 ATU and at 378 ATU higher than at the other groups (p < 0.05 Wilcoxon signed-rank-test). (e) Contraction frequency in dependence of the aggregate size shows no significantly correlation (p = 0.8, t-test). (f) Illustration of contraction frequency of a SCC prepared from a 350 ATU old larva during 30 days observation. Frequency was between 50 and 88 beats/ min. (g) Contraction frequency in dependence of the temperature (n=4, 350 ATU). A highly significant correlation between temperature and contraction frequency was present (p < 0.001, t-test). Higher temperature evokes higher contraction frequencies and vice versa. |
