| (A) Freshly cut vibratome slices of adult rat heart (non-fixed) were stained with DAPI to reveal dead/damaged cells at the periphery of the slice (blue). Cardiomyocytes
were seen with their autofluorescence (green). DAPI-nuclear staining was still restricted to the outer layers of cells in slices which had been cultured for 2
(B) and 3 days
(C) After 6 days in culture
(D) DAPI-nuclear staining was still most pronounced at the periphery of the slice with some extracellular staining
(released DNA from dead cells) but some nuclei were also stained towards the middle of the slice. Please note that the myocardial structure was maintained
throughout the period (A-D). One representative picture from 4 slices (from 4 different hearts) at each time point is presented. Bar markers = 100 μm. |
