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| Figure 3: Immunofluorescence images of undifferentiated type A spermatogonia treated or untreated with Oct-4 shRNA captured on Laser scanning confocal microscope (LSCM). (a) Cells transfected with NSCV showing normal nuclear Oct-4 expression. (b) Undifferentiated type A spermatogonia transfected with NSCV shown by DAPI staining. (c) Undifferentiated type A spermatogonial cells without shRNA Transfection showing nuclear Oct-4 expression. (d) Undifferentiated type A Spermatogonial cells without shRNA transfection shown by DAPI staining. (e) Undifferentiated type A spermatogonia 48 h post transfection with Oct-4 shRNA-2, showing significant knock-down of Oct-4 expression as compared to NSCV treated cells. (f) Undifferentiated type A spermatogonia 48 h post transfection with Oct-4 shRNA-2 shown by DAPI staining (X63). The figure shown is the representative pictures from three independent experiments. |
