| Gene |
Sequence |
Product size |
Annealing Temperature |
| Oct-4 |
Forward 5’-cgggctgggtggatcctcga-3’
Reverse 5’-ttcacggcattggggcggtc-3’ |
277bp |
65°C |
| Plzf |
Forward 5’-gcaggagccagcaaaggcga-3’
Reverse 5’-gcagagaccccagggagggg-3’ |
196bp |
65°C |
| Gfra-1 |
Forward 5’-ggctaggaggaggagatgct-3’
Reverse 5’-ctggatgtgaccagggactt-3’ |
231bp |
65°C |
| c-Ret |
Forward 5’-tggcacacctctgctctatg-3’
Reverse 5’-ctgttcccaggaactgtggt-3’ |
187bp |
58°C |
| Bcl6b |
Forward 5’-gcagcagtgaagaaggaacc-3’
Reverse 5’-agccacagcctcacagttct-3’ |
206bp |
61°C |
| Etv5 |
Forward 5’-gggagagacaaaaaccacca-3’
Reverse 5’-atgggtgtgcagtttcttcc-3’ |
219bp |
62°C |
| Pcna |
Forward 5’-gaacaggagtacagctgtgta-3’
Reverse 5’-caggctcattcatctctatgg-3’ |
220bp |
60°C |
| Pou3f1 |
Forward 5’-cctggggtccttctaactcc-3’
Reverse 5’- ggaggagaggggaagagaaa-3’ |
170bp |
60°C |
| c-kit |
Forward 5’-ttatcctttaggccgtgtgg-3’
Reverse 5’-tgtggccccttaagtacctg-3’ |
230bp |
58°C |
| Scp3 |
Forward 5’-ggggccggactgtatttact-3’
Reverse 5’-aggctgatcaaccaaaggtg-3’ |
169bp |
60°C |
| Gapdh |
Forward 5’-aactttggcattgtggaagg-3’.
Reverse 5’-acacattgggggtaggaaca-3’ |
223bp |
64°C |
