Human monocyte derived DCs were pulsed for three days with DAMPs obtained from necrotic material of 105 lysed HCT-116 colorectal tumor cells/ml, or with S100A4 (1μg/ml) in the absence or presence of anti-S100A4 antibodies. Thus pulsed DCs were co-cultured with autologous lymphocytes for 5 (±1) days. Shown is the increased metabolic activity (mean ± SEM) of lymphocytes compared to lymphocytes co-cultured with non-pulsed autologous DCs. Metabolic activity was assessed by measuring the amount of WST-1 cleaved per hour and increased activity was shown in percent increase. Asterisks indicate p ≤ 0.05.
