PARP-1-N-term-pEGFP construct was introduced into IRK cells by electroporation. After 24 hours live cells were examined using confocal laser scanning microscopy. A. Panels 1 and 2, cells expressing pEGFP plasmid (control). Panels 3 and 4, cells expressing the construct. Scale bar represents 75 μm. Panels 4 and 5, nuclei of cells in interphase expressing the construct. Scale bar represents 10 μm. B. Optical sections of Z stack collected at 0.8 μm interval. C. Mitotic cells expressing the construct. Panels 1-6 are prophase, prometaphase, metaphase, anaphase, early telophase and telophase respectively. Scale bar for B and C represents 10 μm. Each panel is representative of atleast 60 cells from three separate transfections.
