|
| Figure 5: Endogenous PARP-1 expression is reduced in cells expressing PARP-1 N-term-EGFP IRK cells were electroporated with PARP-1-N-term-pEGFP. Cell extracts were prepared at different times after electroporation and analysed by Western blot using antiPARP-1 antibody (raised against a synthetic peptide corresponding to amino acids 2-20 at the N-terminal of PARP-1) followed by peroxidaseconjugated secondary antibody. Lane 1, 24 hours, Lane 2, 48 hours, Lane 3, 72 hours. Lane 4, Non-transfected IRK cell extract (control). Red and blue arrows indicate endogenous PARP-1 and PARP-1-N-term-EGFP bands at 116 kDa and 55 kDa respectively. Data is representative of three separate experiments. |
