Base Medium Serum Glutamine bFGF NGF EGF Insulin Transferrin Others Antibiotics References
MEM 10% HS         5 μg/mL 5 μg/mL 5 ng/mL sodium selenite, 150 g/mL ECGS 100 U/mL penicillin, 100 g/mL streptomycin, 0.25 g/mL amphotericin B Blake et al. 1997
MEM 5% CS+10% FBS 200 mM           2% esssential aminoacids, 1% non essential aminoacids 10 g/mL gentamicin, 1,2 g/mL amphotericin B Yue et al. 1989
Low glucose DMEM 15% FBS   2 ng/mL           2.5 mg/L amphotericin B, 2.5 mg/L doxycycline Yamaguchi et al. 2011
DMEM 10% FBS   2 ng/mL           50 U/mL penicillin, 50 g/mL streptomycin Ishino et al. 2004
DMEM 15% serum 30 mg/L L-glu 2 ng/mL           2.5 mg/L doxycycline, 2.5 mg/L fungizone, 30 mg/L L-glutamine Miyata et al 2001, Amano et al. 2003
DMEM 15% FCS   2 ng/mL         Asc-2P 0.3 mM NM Shima et al. 2011
DMEM/F12 15% FBS       10 ng/mL 5 μg/mL   100 ng/mL bovine pituitary extract 50 mg/mL gentamicin and 1% antibiotic antimycotic solution Jiucheng et al. 2011
DMEM/F12 20% BCS         1% ITS 1% ITS 1% ITS 1% antibiotic-antimycotic Cheng 2007
OPTI-MEM-I 8% FBS     20 ng/mL 5 ng/mL EGF     20 μg/mL ascorbic acid, 200 mg/L calcium chloride, 100 g/mL pituitary extract, 0.08% chondroitin sulfate 1X antibiotic/antimycotic, 5 g/mL gentamicin Zhu et al. 2004, Joyce et al. 2009, Gary et al. 2011, Mimura et al. 2012
EGM-2 10% FBS   Present in BM   Present in BM     IGF, VEGF present in BM Gentamicin, amphotericin B Choi et al. 2010
M199 20% HuS 4 mM           400 μg/mL ECGS 100 g/mL penicillin, 100 g/mL streptomycin Pitsov et al. 1988
F99:Ham’s F12 and M199 (1:1) 5%FBS   10 ng/mL     20 μg/ml 2.5 μg/mL 20 μg/mL ascorbic acid, 0.6 ng/ml sodium selenite NM Engelmann et al. 1995, Engelmann et al. 1989, Gary et al. 2011
EGM: Endothelial Growth Medium; BM: Base Medium; NM: Not Mentioned; MEM: Minimum Essential Medium; DMEM: Dulbecco’s Modified Eagle Medium; HS: Horse Serum; FBS: Fetal Bovine Serum; CS: Calf Serum; FCS: Fetal Calf Serum; BCS: Bovine Calf Serum; HuS: Human serum; bFGF: basic Fibroblast Growth Factor; NGF: Nerve Growth Factor; EGF: Epidermal Growth Factor; IGF: Insulin: Like Growth Factor; VEGF: Vascular Endothelial Growth Factor; ECGS: Endothelial Cell Growth Supplement; ITS: Insulintransferrin-Selenium; Asc-2P: L-Ascorbic Acid-Phosphate.
Table 1: List of different constituents for media preparation used by various researchers for culturing corneal endothelial cells in vitro [10,13,14,16-18,22,30-39].
Goto home»