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| Figure 6: Luciferase expression in 293T cells co-transfected with pTATκ- Oct-3/4 or pTATκ-Sox2 and 6xO/S pGL3. 293T cells were co-transfected with 2.0 μg 6XO/S-Luc luciferase reporter plasmid (containing OCT3/4 and SOX2 binding sites) and either 2.0 μg OCT3/4 or 2.0 μg SOX2; or 1.0 μg OCT3/4 and 1.0 μg SOX2 expression vectors. Un-transfected cells were treated as negative controls. 24 hours after the transfection, cells were lysed with 1X Glo Lysis Buffer (Promega) and incubated for 10 minutes at room temperature. Luciferase activity was measured with the ONE-Glo™ Luciferase Assay system (Promega) and BioTek EL808 Microplate Luminometer detection system, according to the manufacturer’s protocol. Data shown are the mean ± SD of Relative Luciferase Activity (RLA) from 3 independent experiments. |
