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| Figure 8: Analysis of luciferase induction by secreted TATκ-OCT-3/4 protein in 6xO/S pGL3 transfected 293T cells. 293T was transfected with 2.0 μg 6XO/S-Luc luciferase reporter plasmid (containing OCT3/4 and SOX2 binding sites). 24 hours after the transfection, concentrated culture medium containing the secreted proteins; TATκ-OCT3/4 was added to the culture media and incubated for 3 hours. Cells were harvested and lysed with 1X Glo Lysis Buffer (Promega) and incubated for 10 min at room temperature. Luciferase activities were measured with a ONE-Glo™ Luciferase Assay system (Promega) and BioTek EL808 Microplate Luminometer detection system, according to the manufacturer’s protocol. Data shown are the mean ± SD of Relative Luciferase activity (RLA) from 3 independent experiments. |
