| Primer name |
Sequence |
PCR condition |
Fragment length |
| Alb |
For: 5’GGTATGAATATGCAAGAAG3’
Rev: 5’CACTCTTCCCAGGTTTCTTG3’ |
48°C |
350 bp |
| Ck-18 |
For: 5’GGACCTCAGCAAGATCATGGC3’
Rev: 5’CCAGGATCTTACGGGTAGTTG3’ |
50°C |
518 bp |
| Afp |
For: 5’CCCACCCTTCCACTTTCCAGA3’
Rev: 5’GCTGGAACTGCCTTGTCATA3’ |
54°C |
164 bp |
| Gapdh |
For: 5’GAGTTGCTGTTGAAGTCACAGG3’
Rev: 5’CAGCAATGCATCCTGCAC3’ |
42°C |
429 bp |
| Alb- Albumin; Ck-18, Cytokeratin 18; Afp, Alpha-fetoprotein; Gapdh, Glyceraldehyde 3-phosphate dehydrogenase |
