Gene Tm [°C]
theoretical experimental ±SD
GAPDH 88,2 88,3 ±0,1
β-actin 95,6 94,6 ±0,3
Decorin 83,5 83,6 ±0,1
Vimentin 82,2 82,2 ±0,1
Table 2: The comparison of theoretical and experimental melting temperatures of obtained amplicons
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