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Figure 1: GPC3 overexpression in Huh-7 cells after GPC3 transfection. A: Relative expression of GPC3 mRNA in Huh-7 cells transfected with cDNA3.1 (+)-GPC3, blank plasmid (mock), or control. B: Representative western blot bands of GPC3 protein expression in in Huh-7 cells transfected with cDNA3.1 (+)-GPC3, blank plasmid (mock), or control. GAPDH used as a loading control. C: Summarized data of GPC3 protein expression in three groups. Data was expressed as mean ± SD, n=3/group, *P< 0.05, **P< 0.01, versus control or mock. |