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Figure 4: Immunohistochemistry of skeletal muscle section of DIO mouse with anti-AQP9 antibody reveals the presence of scattered myofibers with positive immunostaining at their fiber surface (A). These AQP9 positive myofibers (asterisk) in A are also immunopositive with anti-AQP4 antibody in serial muscle section (asterisk in B), and are type 2 fibers. Control muscle section of chow-fed mouse (C) immunostained with anti-AQP9 antibody shows similar immunostaining appearance to the DIO muscle section (A). Bar=50 μm (A, B, C). |