a. Immunoblotting of proteins relevant to EGFR and COX-2 signaling pathways on untreated SQ20B, SCC61 and SCC25 head and neck squamous cell lines. These levels are compared to WI-38 fibroblasts. Proteins analyzed by immunoblotting are phosphorylated EGFR (p-EGFR)/EGFR, COX-2, phosphorylated AKT (p-AKT)/ AKT, phosphorylated ERK (p-ERK)/ERK, phosphorylated STAT3 (p-STAT3)/STAT3 and actin for control.
b. Cell viability assay assessed by the MTT assay for three HNSCC cell lines, SQ20B, SCC61 and SCC25, treated with erlotinib (0-6 μM) for 24 hours. Each experiment was performed in triplicate. Points represent the mean and error bars represent the standard error.
c. Results for the MTT cell viability assay for SQ20B, SCC61 and SCC25 cells treated with celecoxib (0-100 μM) for 24 hours. Each experiment was performed in triplicate. Points represent the mean and error bars represent the standard error.
